Determination Method of Isocitric Acid in Food Additive Citric Acid.

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Citric Acid Determination

Three types of methods have been proposed for the determination of citric acid in urine. Salant and Wise (1) used the unspecific mercuric sulfate reagent of Deniges (2) in a study of the toxicity of citrates. Thunberg (3) developed an enzymatic method employing a dehydrogenase said to be specific for citrate. The remaining methods depend on the so called pentabromoacetone method of Stahre (4), ...

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Numerous methods, enzymatic and chemical, have been reported for the determination of small amounts of citric acid in biological fluids. Of the chemical methods the majority involve the conversion of the citric acid to pentabromoacetone, which may be estimated by various gravimetric, titrimetric, or calorimetric procedures. The more recent modifications (l-3) of the met.hod have simplified it a...

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Mix**, wait for the end of the reaction (approx. 10 min) and read the absorbances of the solutions (A2). If the reaction has not stopped after 10 min, continue to read the absorbances at 2 min intervals until the absorbance increases constantly over 2 min. Determine the absorbance differences (A2-A1) for both, blank and sample. Subtract the absorbance difference of the blank from the absorbance...

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ژورنال

عنوان ژورنال: Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi)

سال: 2001

ISSN: 0015-6426,1882-1006

DOI: 10.3358/shokueishi.42.45